Hotspot

accD-psaI

LSC · 57872-58319 · 448 bp

Back to Sinocarum

Pi 0.0984

MarkerSeek score 79.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 97
pi: 0.0984
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGAATCAAAATTCAATAGAGCA	AGGGAAAGTCGAAAGTTGTCA	27.3	42.9	52.1	57.4	313-681 bp	0.875	47.0
accD-psaI_p2	TGAATCAAAATTCAATAGAGCAT	AGGGAAAGTCGAAAGTTGTCA	26.1	42.9	52.7	57.4	313-681 bp	0.875	47.0
accD-psaI_p3	TGAATCAAAATTCAATAGAGCATT	AGGGAAAGTCGAAAGTTGTCA	25.0	42.9	53.6	57.4	313-681 bp	0.875	47.0
accD-psaI_p4	TGAATCAAAATTCAATAGAGCA	GGGAAAGTCGAAAGTTGTCA	27.3	45.0	52.1	55.9	312-680 bp	0.875	47.0
accD-psaI_p5	TGAATCAAAATTCAATAGAGCA	AGGGAAAGTCGAAAGTTGTCAT	27.3	40.9	52.1	57.9	313-681 bp	0.875	47.0