Hotspot

ndhC-trnV-UAC

LSC · 49817-50679 · 863 bp

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Pi 0.0401
MarkerSeek score 81.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
863
variable_sites
83
pi
0.0401
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 777-1113 bp 1.0 51.1
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 792-1128 bp 1.0 51.0
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 30.4 55.0 54.5 58.1 793-1129 bp 1.0 51.0
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 30.4 55.0 54.5 58.1 792-1128 bp 1.0 51.0
ndhC-trnV-UAC_p5 CATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 33.3 55.0 52.3 58.1 791-1127 bp 1.0 51.0