Hotspot

rps4-trnT-UGU

LSC · 45590-45932 · 343 bp

Back to Sinocarum

Pi 0.0515

MarkerSeek score 83.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 42
pi: 0.0515
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	457-508 bp	1.0	87.4
rps4-trnT-UGU_p2	GTTAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.7	57.8	425-476 bp	1.0	87.0
rps4-trnT-UGU_p3	TTTTTGAAACGAGGTCCCCG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.7	57.9	424-475 bp	1.0	84.8
rps4-trnT-UGU_p4	GTTAGTCCCGGTAAAGCTCC	CAGAGGTTAGAGCATCGCAT	55.0	50.0	57.7	57.5	455-506 bp	1.0	84.3
rps4-trnT-UGU_p5	GTTAGTCCCGGTAAAGCTCC	TCAGAGGTTAGAGCATCGCA	55.0	50.0	57.7	58.5	456-507 bp	1.0	84.3