Hotspot

trnT-GGU-psbD

LSC · 31534-32219 · 686 bp

Back to Sinocarum

Pi 0.0433
MarkerSeek score 81.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
686
variable_sites
80
pi
0.0433
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC CCAGCCTCCTAAAGCGAAAT 50.0 50.0 58.1 58.0 898-1640 bp 1.0 89.3
trnT-GGU-psbD_p2 AAGGGCCCTTTTAACTCAGC AAACTAGGCGCAAAGAACCA 50.0 45.0 58.1 58.0 1272-2014 bp 1.0 87.7
trnT-GGU-psbD_p3 AAGGGCCCTTTTAACTCAGC AACTAGGCGCAAAGAACCAA 50.0 45.0 58.1 58.0 1271-2013 bp 1.0 87.7
trnT-GGU-psbD_p4 AAGGGCCCTTTTAACTCAGC ACCAAAACTAGGCGCAAAGA 50.0 45.0 58.1 58.0 1276-2018 bp 1.0 87.7
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC CACCAAAACTAGGCGCAAAG 50.0 50.0 58.1 57.9 1277-2019 bp 1.0 87.4