Hotspot

trnK-UUU-rps16

LSC · 4100-4754 · 655 bp

Back to Sinocarum

Pi 0.0507

MarkerSeek score 84.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 83
pi: 0.0507
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	CGATTCAAAGTGTATCGCGC	55.0	50.0	58.0	58.0	1509-1532 bp	0.75	77.5
trnK-UUU-rps16_p2	CCCTTTCAGGATCAGTCGTG	GATTCAAAGTGTATCGCGCG	55.0	50.0	58.0	58.0	1508-1531 bp	0.75	77.5
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CGATTCAAAGTGTATCGCGC	55.0	50.0	58.0	58.0	1508-1531 bp	0.75	77.5
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	GATTCAAAGTGTATCGCGCG	55.0	50.0	58.0	58.0	1507-1530 bp	0.75	77.5
trnK-UUU-rps16_p5	TCCCTCGCTTCATCCAAATG	GATTCAAAGTGTATCGCGCG	50.0	50.0	58.0	58.0	1456-1479 bp	0.75	77.5