Hotspot

infA

LSC · 78995-79336 · 342 bp

Back to Sinobambusa

Pi 0.000975
MarkerSeek score 46.80
Primer available yes
Label infA

Feature Metadata

label_name
infA
feature_type
gene
region
LSC
length_bp
342
variable_sites
1
pi
0.000975
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA_p1 GTCTCTTCGCTTGAATTTTGGA ATGGTAATCGCCCCTCCTAT 40.9 50.0 57.3 57.7 499-517 bp 1.0 58.2
infA_p2 AGTCTCTTCGCTTGAATTTTGG ATGGTAATCGCCCCTCCTAT 40.9 50.0 57.2 57.7 500-518 bp 1.0 58.1
infA_p3 GTCTCTTCGCTTGAATTTTGGA CGCCCCTCCTATAGTACTCG 40.9 60.0 57.3 58.5 491-509 bp 1.0 56.6
infA_p4 AGTCTCTTCGCTTGAATTTTGG CGCCCCTCCTATAGTACTCG 40.9 60.0 57.2 58.5 492-510 bp 1.0 56.6
infA_p5 TCTCTTCGCTTGAATTTTGGAAC ATGGTAATCGCCCCTCCTAT 39.1 50.0 57.9 57.7 498-516 bp 1.0 55.3