Hotspot

ccsA

SSC · 109447-110439 · 993 bp

Back to Sinobambusa

Pi 0.0015

MarkerSeek score 33.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 993
variable_sites: 4
pi: 0.0015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTGGCGGCATTCTCAAAAA	AACTTTCATTTCACGAAGGT	45.0	35.0	58.7	53.3	1099-1114 bp	1.0	41.4
ccsA_p2	AGTGGCGGCATTCTCAAAAA	ACTTTCATTTCACGAAGGTT	45.0	35.0	58.7	53.3	1098-1113 bp	1.0	41.4
ccsA_p3	AGTGGCGGCATTCTCAAAAA	AAACTTTCATTTCACGAAGGT	45.0	33.3	58.7	54.2	1100-1115 bp	1.0	41.4
ccsA_p4	AGTGGCGGCATTCTCAAAAA	AACTTTCATTTCACGAAGGTT	45.0	33.3	58.7	54.2	1099-1114 bp	1.0	41.4
ccsA_p5	AGTGGCGGCATTCTCAAAAA	ACTTTCATTTCACGAAGGTTT	45.0	33.3	58.7	54.2	1098-1113 bp	1.0	41.4