Hotspot

ndhC-trnV-UAC

LSC · 52640-53383 · 744 bp

Back to Sinobambusa

Pi 0.000768

MarkerSeek score 43.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 2
pi: 0.000768
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	877-894 bp	1.0	75.9
ndhC-trnV-UAC_p2	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	881-898 bp	1.0	74.7
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	878-895 bp	1.0	74.0
ndhC-trnV-UAC_p4	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.8	57.9	880-897 bp	1.0	73.2
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.4	58.3	882-899 bp	1.0	72.9