Hotspot

trnT-UGU-trnL-UAA

LSC · 48484-49341 · 858 bp

Back to Sinobambusa

Pi 0
MarkerSeek score 50.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
858
variable_sites
0
pi
0
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 917-959 bp 1.0 83.7
trnT-UGU-trnL-UAA_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 909-951 bp 1.0 83.4
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 887-929 bp 1.0 82.9
trnT-UGU-trnL-UAA_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 907-949 bp 1.0 81.8
trnT-UGU-trnL-UAA_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 916-958 bp 1.0 81.6