Hotspot

psaA-ycf3

LSC · 43941-44744 · 804 bp

Back to Sinobambusa

Pi 0.000355

MarkerSeek score 43.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 1
pi: 0.000355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.5	58.0	950-951 bp	1.0	76.2
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCGG	TCTGAGCCGTATGAGGTAGG	50.0	55.0	58.5	58.0	951-952 bp	1.0	76.2
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.5	58.0	878-879 bp	1.0	76.1
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	TCTGAGCCGTATGAGGTAGG	50.0	55.0	58.5	58.0	879-880 bp	1.0	76.1
psaA-ycf3_p5	CTGGTTCCGGCGAACTAATA	CTGAGCCGTATGAGGTAGGA	50.0	55.0	57.4	58.0	870-871 bp	1.0	75.2