Hotspot

matK-trnK-UUU

LSC · 3332-4016 · 685 bp

Back to Sinobambusa

Pi 0

MarkerSeek score 43.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	47.6	50.0	58.3	57.8	859-861 bp	1.0	68.4
matK-trnK-UUU_p2	ACTCCTGAAAGAGAAGTGGG	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	859-861 bp	1.0	66.5
matK-trnK-UUU_p3	CTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	858-860 bp	1.0	66.5
matK-trnK-UUU_p4	ACTCCTGAAAGAGAAGTGGGT	TGGGTTGCTAACTCAATGGT	47.6	45.0	58.3	57.0	800-802 bp	1.0	62.0
matK-trnK-UUU_p5	ACTCCTGAAAGAGAAGTGGGTA	CGTGCGGTGTAATTCCATTG	45.5	50.0	58.1	57.8	859-861 bp	1.0	61.7