Hotspot

rpl32-trnL

SSC · 112921-113577 · 657 bp

Back to Sinalliaria

Pi 0.0292
MarkerSeek score 65.40
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
657
variable_sites
19
pi
0.0292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 TCGCTTTCTACAGGTAATTCA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 54.7 57.9 741-747 bp 1.0 47.3
rpl32-trnL_p2 TCGCTTTCTACAGGTAATTCA AGCAGCGTGTCTACCAATTT 38.1 45.0 54.7 57.8 734-740 bp 1.0 46.8
rpl32-trnL_p3 TCGCTTTCTACAGGTAATTCA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 54.7 57.8 740-746 bp 1.0 46.7
rpl32-trnL_p4 TCGCTTTCTACAGGTAATTCA CTAAGAGCAGCGTGTCTACC 38.1 55.0 54.7 57.8 739-745 bp 1.0 46.7
rpl32-trnL_p5 TCGCTTTCTACAGGTAATTCA GAGCAGCGTGTCTACCAATT 38.1 50.0 54.7 58.3 735-741 bp 1.0 46.1