Hotspot

rpl14-rpl16

LSC · 80315-80432 · 118 bp

Back to Sinalliaria

Pi 0.0254

MarkerSeek score 67.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 3
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	CAATAGCGGCATCAAAAATGC	50.0	42.9	58.0	57.2	234 bp	1.0	66.0
rpl14-rpl16_p2	ACCAATATGAGCATAGCGGC	CAATAGCGGCATCAAAAATGC	50.0	42.9	58.2	57.2	279 bp	1.0	64.6
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GGTGTACCCGAAAATATAGCCA	50.0	45.5	58.0	57.9	268 bp	1.0	64.0
rpl14-rpl16_p4	ACACATCAATTCTCGAGCCC	TGGTGTACCCGAAAATATAGCC	50.0	45.5	58.0	57.9	269 bp	1.0	64.0
rpl14-rpl16_p5	ATGAGCATAGCGGCGATTAC	CAATAGCGGCATCAAAAATGC	50.0	42.9	58.3	57.2	273 bp	1.0	63.8