Hotspot

rps11-rpl36

LSC · 78617-78727 · 111 bp

Back to Sinalliaria

Pi 0.045

MarkerSeek score 77.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 5
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGAACCACTACGTGTATT	GTTCCAACCCGAGGCATAAA	45.0	50.0	55.5	58.2	202 bp	1.0	61.0
rps11-rpl36_p2	CGTGAACCACTACGTGTATT	TTCCAACCCGAGGCATAAAC	45.0	50.0	55.5	58.2	201 bp	1.0	61.0
rps11-rpl36_p3	CGTGAACCACTACGTGTATT	AAGGGCTTCCGTTCGTAAAA	45.0	45.0	55.5	57.7	273 bp	1.0	59.8
rps11-rpl36_p4	CGTGAACCACTACGTGTATTT	GTTCCAACCCGAGGCATAAA	42.9	50.0	56.3	58.2	202 bp	1.0	59.2
rps11-rpl36_p5	CGTGAACCACTACGTGTATTT	TTCCAACCCGAGGCATAAAC	42.9	50.0	56.3	58.2	201 bp	1.0	59.2