Hotspot

trnP-psaJ

LSC · 65722-66057 · 336 bp

Back to Sinalliaria

Pi 0.0092

MarkerSeek score 49.00

Primer available yes

Label trnP-psaJ

Feature Metadata

label_name: trnP-psaJ
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 3
pi: 0.0092
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-psaJ_p1	ACCCGTGACATTTTGTACCC	GGTGCTACGGAAAGATATGT	50.0	45.0	58.1	55.0	419-426 bp	1.0	57.3
trnP-psaJ_p2	CCCGTGACATTTTGTACCCA	GGTGCTACGGAAAGATATGT	50.0	45.0	58.1	55.0	418-425 bp	1.0	57.3
trnP-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGTGCTACGGAAAGATATGT	50.0	45.0	58.1	55.0	430-437 bp	1.0	57.2
trnP-psaJ_p4	CAGGATTTGAACCCGTGACA	GGTGCTACGGAAAGATATGT	50.0	45.0	58.1	55.0	429-436 bp	1.0	57.2
trnP-psaJ_p5	TGACAGGATTTGAACCCGTG	GGTGCTACGGAAAGATATGT	50.0	45.0	58.1	55.0	432-439 bp	1.0	57.1