Hotspot

cemA-petA

LSC · 60494-60718 · 225 bp

Back to Sinalliaria

Pi 0.0356

MarkerSeek score 69.10

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 8
pi: 0.0356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCGTCACTTGT	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.4	57.2	324 bp	1.0	70.5
cemA-petA_p2	CGTGTATCTCCGTCACTTGT	ATGCACTTGAAATGGATGCC	50.0	45.0	57.4	57.0	379 bp	1.0	68.8
cemA-petA_p3	CGTGTATCTCCGTCACTTGT	TGAGACAGATATGGAGCGAGT	50.0	47.6	57.4	58.1	336 bp	1.0	68.4
cemA-petA_p4	CGTGTATCTCCGTCACTTGT	AGTGAGACAGATATGGAGCGA	50.0	47.6	57.4	58.1	338 bp	1.0	68.3
cemA-petA_p5	TCGTGTATCTCCGTCACTTG	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.1	57.2	325 bp	1.0	68.2