Hotspot

ycf4-cemA

LSC · 59339-59803 · 465 bp

Back to Sinalliaria

Pi 0.0194

MarkerSeek score 67.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 9
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AGGTCAAGGGGCTATTCCTT	AGAGAGATCAACCAGGGCAA	50.0	50.0	58.0	58.3	654-730 bp	1.0	78.2
ycf4-cemA_p2	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.0	58.3	578-654 bp	1.0	78.2
ycf4-cemA_p3	AGGTCAAGGGGCTATTCCTT	TCAACCAGGGCAAAAAGACT	50.0	45.0	58.0	57.5	647-723 bp	1.0	77.3
ycf4-cemA_p4	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	45.0	45.0	58.0	57.5	571-647 bp	1.0	77.3
ycf4-cemA_p5	AGGTCAAGGGGCTATTCCTT	GAGAGATCAACCAGGGCAAA	50.0	50.0	58.0	57.5	653-729 bp	1.0	77.0