Hotspot

trnF-ndhJ

LSC · 47124-47710 · 587 bp

Back to Sinalliaria

Pi 0.0172

MarkerSeek score 64.20

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 10
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	697-699 bp	1.0	80.9
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	696-698 bp	1.0	80.9
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	694-696 bp	1.0	79.3
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	700-702 bp	1.0	78.4
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CTTATGATAGCCATCCGCGG	50.0	55.0	58.1	58.4	735-737 bp	1.0	78.3