Hotspot

trnE-trnT

LSC · 29976-30785 · 810 bp

Back to Sinalliaria

Pi 0.0247

MarkerSeek score 63.90

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 20
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	951-966 bp	1.0	80.3
trnE-trnT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	930-945 bp	1.0	79.9
trnE-trnT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	927-942 bp	1.0	79.7
trnE-trnT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	913-928 bp	1.0	79.6
trnE-trnT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	892-907 bp	1.0	79.5