Hotspot

rpoC1-rpoB

LSC · 22536-22561 · 26 bp

Back to Sinalliaria

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TTTTAGTGGCCCAAGCACTT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.9	57.9	181 bp	1.0	79.0
rpoC1-rpoB_p2	TTTTTAGTGGCCCAAGCACT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.9	57.9	182 bp	1.0	79.0
rpoC1-rpoB_p3	AGTGGCCCAAGCACTTATTT	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.7	57.9	177 bp	1.0	77.2
rpoC1-rpoB_p4	GGGTTTTGTCACCTCTCCAA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	222 bp	1.0	76.7
rpoC1-rpoB_p5	GTGGCCCAAGCACTTATTTG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	176 bp	1.0	76.7