Hotspot

ycf1

SSC · 113559-119246 · 5688 bp

Back to Simarouba

Pi 0.317

MarkerSeek score 74.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5688
variable_sites: 1503
pi: 0.317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATCAGTTCGCTCAAACCA	TTTTCTCCAAAGGGTGTCCG	50.0	50.0	58.2	58.0	709 bp	0.5	58.3
ycf1_p2	TGGATCAGTTCGCTCAAACC	TTTTCTCCAAAGGGTGTCCG	50.0	50.0	58.2	58.0	710 bp	0.5	58.3
ycf1_p3	GGATCAGTTCGCTCAAACCA	AAAGGGTGTCCGCTCTTTTT	50.0	45.0	58.2	57.9	701 bp	0.5	57.9
ycf1_p4	TGGATCAGTTCGCTCAAACC	AAAGGGTGTCCGCTCTTTTT	50.0	45.0	58.2	57.9	702 bp	0.5	57.9
ycf1_p5	ACCTGGAGCTTTGGTTCTTC	TTTTCTCCAAAGGGTGTCCG	50.0	50.0	57.7	58.0	963 bp	0.5	57.6