Hotspot

trnL-UAG

SSC · 129037-129116 · 80 bp

Back to Simarouba

Pi 0.494

MarkerSeek score 76.10

Primer available yes

Label trnL-UAG

Feature Metadata

label_name: trnL-UAG
feature_type: tRNA
region: SSC
length_bp: 80
variable_sites: 38
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG_p1	ACTTGGAGAGCGGGTACTAA	AACCGGTACAACGATTCGAG	50.0	50.0	57.8	58.0	822 bp	0.5	58.1
trnL-UAG_p2	ACTTGGAGAGCGGGTACTAA	CCGGTACAACGATTCGAGTT	50.0	50.0	57.8	58.0	820 bp	0.5	58.1
trnL-UAG_p3	ACTTGGAGAGCGGGTACTAA	TGGGTAGCGTTGAAAGCTTT	50.0	45.0	57.8	58.0	1313 bp	0.5	58.0
trnL-UAG_p4	ACTTGGAGAGCGGGTACTAA	TTGGGTAGCGTTGAAAGCTT	50.0	45.0	57.8	58.0	1314 bp	0.5	58.0
trnL-UAG_p5	GACTTGGAGAGCGGGTACTA	CCGGTACAACGATTCGAGTT	55.0	50.0	58.2	58.0	821 bp	0.5	58.0