Hotspot

ndhI

SSC · 123522-124034 · 513 bp

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Pi 0.465

MarkerSeek score 80.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 513
variable_sites: 214
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GTTGATATAGTTGGGGCGCA	TGAACCGACCGGGAATTTTT	50.0	45.0	58.0	58.0	1444 bp	0.5	59.6
ndhI_p2	GTTGATATAGTTGGGGCGCA	TTGAACCGACCGGGAATTTT	50.0	45.0	58.0	58.0	1445 bp	0.5	59.6
ndhI_p3	GTTGATATAGTTGGGGCGCA	ACCCCATCACCAATAGTCCA	50.0	50.0	58.0	58.0	1795 bp	0.5	59.5
ndhI_p4	GTTGATATAGTTGGGGCGCA	TAATCCCGTACCACGACGTA	50.0	50.0	58.0	58.0	1869 bp	0.5	59.4
ndhI_p5	GTTGATATAGTTGGGGCGCA	ACGGAACCCTAGGAATTGGA	50.0	50.0	58.0	58.0	1141 bp	0.5	59.4