Hotspot

ndhH

SSC · 120013-121194 · 1182 bp

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Pi 0.49

MarkerSeek score 80.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 470
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCCAACGTCTGCTAGCTTAT	AGCCTCAATCCACCCTTACT	50.0	50.0	58.1	58.0	2128 bp	0.5	59.3
ndhH_p2	GCCAACGTCTGCTAGCTTAT	TGAGCCTCAATCCACCCTTA	50.0	50.0	58.1	58.0	2130 bp	0.5	59.3
ndhH_p3	GCCAACGTCTGCTAGCTTAT	CCTCAATCCACCCTTACTGC	50.0	55.0	58.1	58.0	2126 bp	0.5	59.2
ndhH_p4	GCCAACGTCTGCTAGCTTAT	GCCTCAATCCACCCTTACTG	50.0	55.0	58.1	58.0	2127 bp	0.5	59.2
ndhH_p5	TGCGGATCGGTTGAATTTCA	AGCCTCAATCCACCCTTACT	45.0	50.0	58.2	58.0	2252 bp	0.5	58.3