Hotspot

ndhF

SSC · 130215-132434 · 2220 bp

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Pi 0.459

MarkerSeek score 79.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 878
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAGCTTTCAACGCTACCCA	ATCCAGCAAGTTTGGCAACT	45.0	45.0	58.0	58.3	2812 bp	0.5	57.5
ndhF_p2	AAGCTTTCAACGCTACCCAA	ATCCAGCAAGTTTGGCAACT	45.0	45.0	58.0	58.3	2811 bp	0.5	57.5
ndhF_p3	CAACGCTACCCAATATCCCC	ATCCAGCAAGTTTGGCAACT	55.0	45.0	58.1	58.3	2804 bp	0.5	56.8
ndhF_p4	TCGAGTCAGGCTGATTCAGA	ATCCAGCAAGTTTGGCAACT	50.0	45.0	58.2	58.3	2920 bp	0.5	56.4
ndhF_p5	GCTACCCAATATCCCCGTTC	ATCCAGCAAGTTTGGCAACT	55.0	45.0	57.8	58.3	2800 bp	0.5	56.2