Hotspot

ndhE

SSC · 125214-125519 · 306 bp

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Pi 0.446

MarkerSeek score 73.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 112
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGGACTATTGGTGATGGGGT	TTAAAGAACCCGCGGCTATC	50.0	50.0	58.0	58.1	1539 bp	0.5	59.4
ndhE_p2	TGGACTATTGGTGATGGGGT	TGCTTTACATCCGTCCCAAG	50.0	50.0	58.0	57.9	1174 bp	0.5	59.0
ndhE_p3	TGGACTATTGGTGATGGGGT	CTTACAACCAACGCAGTCCT	50.0	50.0	58.0	58.1	1222 bp	0.5	58.9
ndhE_p4	TGGACTATTGGTGATGGGGT	ACATCCGTCCCAAGGTATCA	50.0	50.0	58.0	58.1	1168 bp	0.5	58.8
ndhE_p5	TGGACTATTGGTGATGGGGT	TTACAACCAACGCAGTCCTC	50.0	50.0	58.0	58.1	1221 bp	0.5	58.8