Hotspot

ndhD

SSC · 126142-127671 · 1530 bp

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Pi 0.49

MarkerSeek score 81.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 644
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCGTGTCCCACAGATGTATT	GCTAATGAGGCATGGGGATC	50.0	55.0	58.0	58.2	2380 bp	0.5	58.5
ndhD_p2	GCGTGTCCCACAGATGTATT	GGGCGTCTGGTCACAATTAA	50.0	50.0	58.0	58.2	2016 bp	0.5	58.3
ndhD_p3	GCGTGTCCCACAGATGTATT	GGGAGCAGTATGGGCTAATG	50.0	55.0	58.0	57.8	2393 bp	0.5	58.3
ndhD_p4	GCGTGTCCCACAGATGTATT	TTCCGCAATTGTGGCTTCTA	50.0	45.0	58.0	57.8	2258 bp	0.5	58.3
ndhD_p5	CTTGGGACGGATGTAAAGCA	GCTAATGAGGCATGGGGATC	50.0	55.0	57.9	58.2	2349 bp	0.5	57.8