Hotspot

ccsA

SSC · 127969-128928 · 960 bp

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Pi 0.476

MarkerSeek score 82.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 423
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGGACTGGCTATCCCAATTC	GTGAAATCGGTAGACACGCT	55.0	50.0	58.1	58.0	1845 bp	0.5	59.0
ccsA_p2	CGGACTGGCTATCCCAATTC	TGAAATCGGTAGACACGCTG	55.0	50.0	58.1	58.0	1844 bp	0.5	59.0
ccsA_p3	CGGACTGGCTATCCCAATTC	AACCGGTACAACGATTCGAG	55.0	50.0	58.1	58.0	2090 bp	0.5	59.0
ccsA_p4	CGGACTGGCTATCCCAATTC	CCGGTACAACGATTCGAGTT	55.0	50.0	58.1	58.0	2088 bp	0.5	59.0
ccsA_p5	TTATCTCGACGAAATGGGCG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.2	58.0	1863 bp	0.5	58.7