Hotspot

rpl32-ndhF

SSC · 129919-130214 · 296 bp

Back to Simarouba

Pi 0.419

MarkerSeek score 76.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 296
variable_sites: 114
pi: 0.419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AACTCGAATCGTTGTACCGG	AAAGTAGAAGCCCCACTCCT	50.0	50.0	58.0	58.0	969 bp	0.5	59.5
rpl32-ndhF_p2	CTCGAATCGTTGTACCGGTT	AAAGTAGAAGCCCCACTCCT	50.0	50.0	58.0	58.0	967 bp	0.5	59.5
rpl32-ndhF_p3	AACTCGAATCGTTGTACCGG	TGTTGCCGACGGAAAAAGTA	50.0	45.0	58.0	58.0	983 bp	0.5	59.5
rpl32-ndhF_p4	CTCGAATCGTTGTACCGGTT	TGTTGCCGACGGAAAAAGTA	50.0	45.0	58.0	58.0	981 bp	0.5	59.5
rpl32-ndhF_p5	CTCGAATCGTTGTACCGGTT	TACATGAAGGGGGAATTGCG	50.0	50.0	58.0	57.9	1623 bp	0.5	59.5