Hotspot

trnL-UAG-rpl32

SSC · 129117-129735 · 619 bp

Back to Simarouba

Pi 0.436

MarkerSeek score 80.90

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 619
variable_sites: 264
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	TGGGTAGCGTTGAAAGCTTT	50.0	45.0	58.0	58.0	756 bp	0.5	59.7
trnL-UAG-rpl32_p2	AGCGTGTCTACCGATTTCAC	TTGGGTAGCGTTGAAAGCTT	50.0	45.0	58.0	58.0	757 bp	0.5	59.7
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	TGGGTAGCGTTGAAAGCTTT	50.0	45.0	58.0	58.0	757 bp	0.5	59.7
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	TTGGGTAGCGTTGAAAGCTT	50.0	45.0	58.0	58.0	758 bp	0.5	59.7
trnL-UAG-rpl32_p5	AGCGTGTCTACCGATTTCAC	AAAGTAGAAGCCCCACTCCT	50.0	50.0	58.0	58.0	1212 bp	0.5	59.6