Hotspot

ndhD-ccsA

SSC · 127672-127968 · 297 bp

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Pi 0.526

MarkerSeek score 76.60

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 297
variable_sites: 144
pi: 0.526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GAAGTATTACCGCGTGCTCA	GCTAATGAGGCATGGGGATC	50.0	55.0	58.1	58.2	1227 bp	0.5	58.0
ndhD-ccsA_p2	GAAGTATTACCGCGTGCTCA	GGGAGCAGTATGGGCTAATG	50.0	55.0	58.1	57.8	1240 bp	0.5	57.8
ndhD-ccsA_p3	GAAGTATTACCGCGTGCTCA	TTCCGCAATTGTGGCTTCTA	50.0	45.0	58.1	57.8	1105 bp	0.5	57.8
ndhD-ccsA_p4	CGGACTGGCTATCCCAATTC	GCTAATGAGGCATGGGGATC	55.0	55.0	58.1	58.2	931 bp	0.5	57.7
ndhD-ccsA_p5	CGGACTGGCTATCCCAATTC	GGGAGCAGTATGGGCTAATG	55.0	55.0	58.1	57.8	944 bp	0.5	57.5