Hotspot

ndhI-ndhG

SSC · 124035-124408 · 374 bp

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Pi 0.46

MarkerSeek score 77.80

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 156
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	GTCAACAAACAATACGCGCA	ACCCCATCACCAATAGTCCA	45.0	50.0	58.0	58.0	1155 bp	0.5	59.6
ndhI-ndhG_p2	GTCAACAAACAATACGCGCA	TAATCCCGTACCACGACGTA	45.0	50.0	58.0	58.0	1229 bp	0.5	59.5
ndhI-ndhG_p3	ACGACCGTCACGAATTGAAT	ACCCCATCACCAATAGTCCA	45.0	50.0	57.9	58.0	801 bp	0.5	59.2
ndhI-ndhG_p4	ACGACCGTCACGAATTGAAT	TAATCCCGTACCACGACGTA	45.0	50.0	57.9	58.0	875 bp	0.5	59.2
ndhI-ndhG_p5	TGTGAAGTATGTGTTCGCGT	ACCCCATCACCAATAGTCCA	45.0	50.0	57.9	58.0	991 bp	0.5	58.7