Hotspot

ndhI

SSC · 117368-117868 · 501 bp

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Pi 0.51

MarkerSeek score 81.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 241
pi: 0.51
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGCCGAGTGTGAACGATTAC	AGACCACGGAAGTTACTCCA	50.0	50.0	58.0	58.0	1682 bp	0.5	59.9
ndhI_p2	AGCCGAGTGTGAACGATTAC	AGACTTGTACAGACCACGGA	50.0	50.0	58.0	58.0	1692 bp	0.5	59.9
ndhI_p3	TTTGTGACCTTTGAGGAGCC	AGACCACGGAAGTTACTCCA	50.0	50.0	58.0	58.0	1888 bp	0.5	59.8
ndhI_p4	TTTGTGACCTTTGAGGAGCC	AGACTTGTACAGACCACGGA	50.0	50.0	58.0	58.0	1898 bp	0.5	59.8
ndhI_p5	GACCTTTGAGGAGCCGTATG	AGACCACGGAAGTTACTCCA	55.0	50.0	58.1	58.0	1883 bp	0.5	59.5