Hotspot

ndhG

SSC · 118238-118768 · 531 bp

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Pi 0.468

MarkerSeek score 77.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 198
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TTGATCACATCCGAGCGTTT	CGATGAGTTCCCCGAGTTTT	45.0	50.0	57.9	57.9	1935 bp	0.5	58.5
ndhG_p2	TTGATCACATCCGAGCGTTT	GAATCGATGAGTTCCCCGAG	45.0	55.0	57.9	57.9	1939 bp	0.5	58.1
ndhG_p3	TCAAATTGCTTTGGGTCGGT	CGATGAGTTCCCCGAGTTTT	45.0	50.0	58.3	57.9	1660 bp	0.5	56.8
ndhG_p4	AATTGATCACATCCGAGCGT	CGATGAGTTCCCCGAGTTTT	45.0	50.0	57.7	57.9	1937 bp	0.5	56.7
ndhG_p5	ATTGATCACATCCGAGCGTT	CGATGAGTTCCCCGAGTTTT	45.0	50.0	57.7	57.9	1936 bp	0.5	56.7