Hotspot

ndhF

SSC · 124735-126966 · 2232 bp

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Pi 0.45

MarkerSeek score 76.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 746
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGGTAAGATCCGGCGAATC	GCCGAGAAGAAGGTATCAGC	55.0	55.0	58.2	58.1	2752 bp	0.5	57.2
ndhF_p2	CAGGTAAGATCCGGCGAATC	CTAGCACTGGGTAGACCTCA	55.0	55.0	58.2	57.9	2668 bp	0.5	57.1
ndhF_p3	CAGGTAAGATCCGGCGAATC	TCGTTGTGGTCGGACTCTAT	55.0	50.0	58.2	58.2	2856 bp	0.5	56.9
ndhF_p4	CAGGTAAGATCCGGCGAATC	ATCAATTCGGTCGTTGTGGT	55.0	45.0	58.2	57.8	2866 bp	0.5	56.8
ndhF_p5	CAGGTAAGATCCGGCGAATC	AGAAGAAGGAGCCGAGAAGA	55.0	50.0	58.2	57.8	2762 bp	0.5	56.5