Hotspot

ndhD

SSC · 119895-121397 · 1503 bp

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Pi 0.492

MarkerSeek score 81.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 639
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	AGCAAATTCCGCAATTGTGG	50.0	45.0	58.0	57.9	2128 bp	0.5	59.2
ndhD_p2	AAAACTCGGGGAACTCATCG	AGCAAATTCCGCAATTGTGG	50.0	45.0	57.9	57.9	2356 bp	0.5	58.6
ndhD_p3	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.0	57.8	2259 bp	0.5	58.5
ndhD_p4	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	50.0	50.0	58.0	57.8	2260 bp	0.5	58.5
ndhD_p5	TGATACCTTGGGACGGATGT	AGCAAATTCCGCAATTGTGG	50.0	45.0	58.1	57.9	2166 bp	0.5	58.3