Hotspot

rps15-ndhH

SSC · 113810-113938 · 129 bp

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Pi 0.5

MarkerSeek score 76.50

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 129
variable_sites: 59
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	GCAAGTAACCCAATGCACAC	GTAAATCAGCAGCGACTCCA	50.0	50.0	57.9	58.0	1169 bp	0.5	59.4
rps15-ndhH_p2	GCAAGTAACCCAATGCACAC	TAAATCAGCAGCGACTCCAC	50.0	50.0	57.9	58.0	1168 bp	0.5	59.4
rps15-ndhH_p3	GCAAGTAACCCAATGCACAC	TGAGGTAAATCAGCAGCGAC	50.0	50.0	57.9	58.0	1173 bp	0.5	59.4
rps15-ndhH_p4	TTTCTGCTGTCCGTGATCTG	GTAAATCAGCAGCGACTCCA	50.0	50.0	57.9	58.0	1207 bp	0.5	59.2
rps15-ndhH_p5	TTTCTGCTGTCCGTGATCTG	TAAATCAGCAGCGACTCCAC	50.0	50.0	57.9	58.0	1206 bp	0.5	59.2