Hotspot

ycf1

IRb · 107947-109809 · 1863 bp

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Pi 0.122
MarkerSeek score 65.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
1863
variable_sites
623
pi
0.122
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.70
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GATCAAAGCGAGCCTCTTCA AGAGAGTTGGCCGAGTTAGT 50.0 50.0 58.0 58.1 80-2552 bp 0.310345 63.7
ycf1_p2 CGGGAGAATCAATGACTGCA AGAGAGTTGGCCGAGTTAGT 50.0 50.0 58.0 58.1 80-2779 bp 0.310345 63.6
ycf1_p3 ACGCCAGACTCGATCTCATA AGAGAGTTGGCCGAGTTAGT 50.0 50.0 58.0 58.1 80-2425 bp 0.310345 63.6
ycf1_p4 GCGAGCCTCTTCATTCTGAA AGAGAGTTGGCCGAGTTAGT 50.0 50.0 58.0 58.1 80-2545 bp 0.206897 59.6
ycf1_p5 AAGCGAGCCTCTTCATTCTG AGAGAGTTGGCCGAGTTAGT 50.0 50.0 58.0 58.1 80-2547 bp 0.206897 59.5