Hotspot

clpP1

LSC · 70066-72096 · 2031 bp

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Pi 0.0697
MarkerSeek score 80.90
Primer available yes
Label clpP1

Feature Metadata

label_name
clpP1
feature_type
gene
region
LSC
length_bp
2031
variable_sites
941
pi
0.0697
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1_p1 CGTTAACGCTACCAATGGGA GCCGGGATCATTCAATACGA 50.0 50.0 58.0 57.8 80-2995 bp 0.965517 89.2
clpP1_p2 CGTTAACGCTACCAATGGGA ATACCAAGGCAAACCCATGG 50.0 50.0 58.0 58.1 80-2962 bp 0.931034 88.1
clpP1_p3 CTAGTACATGTTCCTCGGCG CCTGTAATACTCCAACCGCC 55.0 55.0 57.9 58.1 80-2975 bp 0.931034 87.9
clpP1_p4 CTAGTACATGTTCCTCGGCG CTGTAATACTCCAACCGCCC 55.0 55.0 57.9 58.1 80-2974 bp 0.931034 87.9
clpP1_p5 CGTTAACGCTACCAATGGGA GCACCCCTTTTGTTCAATGG 50.0 50.0 58.0 57.8 80-2869 bp 0.931034 87.8