Hotspot

ycf1-trnN-GUU

IRa · 129341-129696 · 356 bp

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Pi 0.109
MarkerSeek score 61.40
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
356
variable_sites
191
pi
0.109
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.82
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 AGGAAGAGATAAGAGGGCCC GATCAAAGCGAGCCTCTTCA 55.0 50.0 57.9 58.0 80-915 bp 0.931034 88.2
ycf1-trnN-GUU_p2 GGAAGAGATAAGAGGGCCCT GATCAAAGCGAGCCTCTTCA 55.0 50.0 57.9 58.0 80-914 bp 0.931034 88.2
ycf1-trnN-GUU_p3 AGGAAGAGATAAGAGGGCCC GCGAGCCTCTTCATTCTGAA 55.0 50.0 57.9 58.0 80-908 bp 0.344828 64.8
ycf1-trnN-GUU_p4 GGAAGAGATAAGAGGGCCCT GCGAGCCTCTTCATTCTGAA 55.0 50.0 57.9 58.0 80-907 bp 0.344828 64.8
ycf1-trnN-GUU_p5 GGAAGAGATAAGAGGGCCCT AAGCGAGCCTCTTCATTCTG 55.0 50.0 57.9 58.0 80-909 bp 0.344828 64.7