Hotspot

rpl32-trnL-UAG

SSC · 112989-113856 · 868 bp

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Pi 0.0622
MarkerSeek score 82.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
868
variable_sites
230
pi
0.0622
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAAGTACTCAAAACTTGGAA CTCGCACTGCTTCCTAAGAG 27.3 55.0 52.3 58.1 80-973 bp 0.931034 49.2
rpl32-trnL-UAG_p2 AAAAAGTACTCAAAACTTGGAA CTCTCGCACTGCTTCCTAAG 27.3 55.0 52.3 58.1 80-975 bp 0.931034 49.2
rpl32-trnL-UAG_p3 AAAAAGTACTCAAAACTTGGAA GCTTCCTAAGAGCAACGTGT 27.3 50.0 52.3 58.2 80-965 bp 0.931034 49.2
rpl32-trnL-UAG_p4 AAAAAGTACTCAAAACTTGGAA TGCTTCCTAAGAGCAACGTG 27.3 50.0 52.3 58.2 80-966 bp 0.931034 49.2
rpl32-trnL-UAG_p5 AAAAGTACTCAAAACTTGGAAT CTCGCACTGCTTCCTAAGAG 27.3 55.0 52.1 58.1 80-972 bp 0.931034 49.2