Hotspot

ndhF-rpl32

SSC · 112052-112814 · 763 bp

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Pi 0.0472
MarkerSeek score 80.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
763
variable_sites
191
pi
0.0472
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGCGTGTAAACCAAAAACC AAAAGCTTTTAACGCTGCCC 50.0 45.0 58.1 57.9 80-1446 bp 0.172414 51.0
ndhF-rpl32_p2 GGGGCGTGTAAACCAAAAAC AAAAGCTTTTAACGCTGCCC 50.0 45.0 58.1 57.9 80-1447 bp 0.172414 51.0
ndhF-rpl32_p3 ATGAAGGGGAAATTGGGCAG AAAAGCTTTTAACGCTGCCC 50.0 45.0 58.1 57.9 80-1681 bp 0.172414 50.8
ndhF-rpl32_p4 CACGAAAATCGAAACTGCCC AAAAGCTTTTAACGCTGCCC 50.0 45.0 58.0 57.9 80-1532 bp 0.137931 47.2
ndhF-rpl32_p5 CACGAAAATCGAAACTGCCC GCCCAATACCCCTTCTGTTT 50.0 50.0 58.0 57.8 80-1462 bp 0.068966 43.3