Hotspot

petA-psbJ

LSC · 62757-63719 · 963 bp

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Pi 0.0431
MarkerSeek score 81.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
963
variable_sites
199
pi
0.0431
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGCGTACAAGGTCTTTTGT ATTCTGGATTGGGCTCATCG 45.0 50.0 58.1 57.7 80-1129 bp 1.0 89.4
petA-psbJ_p2 CCATTACGCGTACAAGGTCT ATTCTGGATTGGGCTCATCG 50.0 50.0 57.7 57.7 80-1134 bp 1.0 87.6
petA-psbJ_p3 CCCATTACGCGTACAAGGTC ATTCTGGATTGGGCTCATCG 55.0 50.0 58.7 57.7 80-1135 bp 1.0 84.1
petA-psbJ_p4 GACCCATTACGCGTACAAGG ATTCTGGATTGGGCTCATCG 55.0 50.0 58.7 57.7 80-1137 bp 1.0 84.1
petA-psbJ_p5 ACGCGTACAAGGTCTTTTGT TGGATTGGGCTCATCGCTAT 45.0 50.0 58.1 58.9 80-1125 bp 0.965517 82.6