Hotspot

trnK-UUU-rps16

LSC · 4174-4834 · 661 bp

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Pi 0.0656
MarkerSeek score 79.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
661
variable_sites
171
pi
0.0656
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 80-913 bp 1.0 90.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 80-912 bp 1.0 90.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 80-1192 bp 1.0 90.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 80-1191 bp 1.0 90.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCTATCCCAATGAGCCGTCT 50.0 50.0 58.2 57.9 80-985 bp 0.965517 88.6