primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4174	4834	80-913	1.000000	90.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4174	4834	80-912	1.000000	90.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.2	57.9	20.3	18.8	0.244399	4174	4834	80-1192	1.000000	90.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.2	57.9	20.3	18.8	0.244399	4174	4834	80-1191	1.000000	90.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.256524	4174	4834	80-985	0.965517	88.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5924	6619	80-810	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5924	6619	80-821	1.000000	89.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	5924	6619	80-840	1.000000	89.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.375253	5924	6619	80-851	1.000000	89.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	ATAGAAATCGAAGTGGGGCG	20	20	45.0	50.0	58.1	57.8	8.1	0.0	0.379817	5924	6619	80-871	1.000000	89.0
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7178	7616	80-618	1.000000	91.0
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7178	7616	80-619	1.000000	91.0
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	20	20	50.0	50.0	57.9	58.3	1.4	0.7	0.348988	7178	7616	80-620	1.000000	89.2
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7178	7616	80-615	1.000000	89.0
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7178	7616	80-616	1.000000	89.0
petN-psbM_p1	petN-psbM	1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	20	20	50.0	40.0	58.3	56.4	0.0	0.0	1.818304	27811	28359	80-694	0.965517	76.1
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	20	20	50.0	40.0	57.6	56.4	0.0	0.0	1.908233	27811	28359	80-632	0.965517	75.4
petN-psbM_p3	petN-psbM	3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	20	20	50.0	40.0	58.3	56.2	0.0	0.0	2.049275	27811	28359	80-693	0.965517	74.2
petN-psbM_p4	petN-psbM	4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	20	20	50.0	40.0	58.3	56.2	0.0	0.0	2.049275	27811	28359	80-692	0.965517	74.2
petN-psbM_p5	petN-psbM	5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	20	21	50.0	38.1	58.3	57.2	0.0	0.0	2.071244	27811	28359	80-695	0.965517	74.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGATGTCCTGAACCGCTAGA	ACTCTATTCCGCGTGCAAAT	20	20	50.0	45.0	57.9	58.0	0.0	4.9	0.169573	29595	30150	80-778	0.965517	89.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	29595	30150	80-687	0.965517	88.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29595	30150	80-710	0.931034	88.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29595	30150	80-708	0.931034	88.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGAGATGTCCTGAACCGCTA	ACTCTATTCCGCGTGCAAAT	20	20	50.0	45.0	57.9	58.0	0.0	4.9	0.169573	29595	30150	80-780	0.931034	87.9
psaA-ycf3_p1	psaA-ycf3	1	GGCACTAGGTCCAATGTGAG	ATTGCAGAGGCTTGGTTTGA	20	20	55.0	45.0	58.0	58.0	11.5	0.0	0.030190	40306	41299	80-1639	1.000000	91.8
psaA-ycf3_p2	psaA-ycf3	2	GGCACTAGGTCCAATGTGAG	TGAAATTGCAGAGGCTTGGT	20	20	55.0	45.0	58.0	58.0	11.5	0.0	0.030190	40306	41299	80-1643	1.000000	91.8
psaA-ycf3_p3	psaA-ycf3	3	GGCACTAGGTCCAATGTGAG	TTGCAGAGGCTTGGTTTGAT	20	20	55.0	45.0	58.0	58.0	11.5	0.0	0.030190	40306	41299	80-1638	1.000000	91.8
psaA-ycf3_p4	psaA-ycf3	4	GCATCAGCATGTAGGTTCCA	ATTGCAGAGGCTTGGTTTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043038	40306	41299	80-1461	1.000000	91.7
psaA-ycf3_p5	psaA-ycf3	5	GCATCAGCATGTAGGTTCCA	TTGCAGAGGCTTGGTTTGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043038	40306	41299	80-1460	1.000000	91.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGTCCAGAAAATGTCATATTCA	GGTTCGAATCCGTATAGCCC	22	20	31.8	55.0	53.4	57.9	0.0	0.0	6.634176	49680	50794	80-1173	0.931034	49.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGTCCAGAAAATGTCATATTCA	CGGTTCGAATCCGTATAGCC	22	20	31.8	55.0	53.4	58.3	0.0	22.8	6.863558	49680	50794	80-1174	0.931034	49.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGTCCAGAAAATGTCATATTCA	ACTGCATGTTGGGTCTTTGA	22	20	31.8	45.0	53.4	57.6	0.0	0.0	6.921504	49680	50794	80-1254	0.931034	49.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AAAAATGTCCAGAAAATGTCA	GGTTCGAATCCGTATAGCCC	21	20	28.6	55.0	52.1	57.9	0.0	0.0	6.988325	49680	50794	80-1178	0.931034	49.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGTCCAGAAAATGTCATATTCAT	GGTTCGAATCCGTATAGCCC	23	20	30.4	55.0	54.1	57.9	0.0	0.0	7.021259	49680	50794	80-1173	0.931034	49.2
accD_p1	accD	1	AATTTGACATAGCGCGGGAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.3	58.0	8.8	26.9	0.281771	56736	58673	80-2534	0.931034	87.0
accD_p2	accD	2	ATTTGACATAGCGCGGGAAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.3	58.0	8.8	26.9	0.281771	56736	58673	80-2533	0.931034	87.0
accD_p3	accD	3	TCCCCAATGAATCCTACGGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	0.0	26.9	0.211091	56736	58673	80-2787	0.896552	86.2
accD_p4	accD	4	TCCCCAATGAATCCTACGGA	ACAGAGATTTTGGCCACAGG	20	20	50.0	50.0	57.8	58.1	0.0	3.9	0.268947	56736	58673	80-2850	0.896552	85.7
accD_p5	accD	5	AAATTTGACATAGCGCGGGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.3	58.0	8.8	26.9	0.281771	56736	58673	80-2535	0.896552	85.6
petA-psbJ_p1	petA-psbJ	1	ACGCGTACAAGGTCTTTTGT	ATTCTGGATTGGGCTCATCG	20	20	45.0	50.0	58.1	57.7	11.7	0.0	0.323143	62757	63719	80-1129	1.000000	89.4
petA-psbJ_p2	petA-psbJ	2	CCATTACGCGTACAAGGTCT	ATTCTGGATTGGGCTCATCG	20	20	50.0	50.0	57.7	57.7	20.4	0.0	0.556135	62757	63719	80-1134	1.000000	87.6
petA-psbJ_p3	petA-psbJ	3	CCCATTACGCGTACAAGGTC	ATTCTGGATTGGGCTCATCG	20	20	55.0	50.0	58.7	57.7	20.4	0.0	0.983438	62757	63719	80-1135	1.000000	84.1
petA-psbJ_p4	petA-psbJ	4	GACCCATTACGCGTACAAGG	ATTCTGGATTGGGCTCATCG	20	20	55.0	50.0	58.7	57.7	20.4	0.0	0.983438	62757	63719	80-1137	1.000000	84.1
petA-psbJ_p5	petA-psbJ	5	ACGCGTACAAGGTCTTTTGT	TGGATTGGGCTCATCGCTAT	20	20	45.0	50.0	58.1	58.9	11.7	0.0	0.996870	62757	63719	80-1125	0.965517	82.6
clpP1_p1	clpP1	1	CGTTAACGCTACCAATGGGA	GCCGGGATCATTCAATACGA	20	20	50.0	50.0	58.0	57.8	16.0	2.2	0.176755	70066	72096	80-2995	0.965517	89.2
clpP1_p2	clpP1	2	CGTTAACGCTACCAATGGGA	ATACCAAGGCAAACCCATGG	20	20	50.0	50.0	58.0	58.1	16.0	4.1	0.148268	70066	72096	80-2962	0.931034	88.1
clpP1_p3	clpP1	3	CTAGTACATGTTCCTCGGCG	CCTGTAATACTCCAACCGCC	20	20	55.0	55.0	57.9	58.1	2.3	0.0	0.171651	70066	72096	80-2975	0.931034	87.9
clpP1_p4	clpP1	4	CTAGTACATGTTCCTCGGCG	CTGTAATACTCCAACCGCCC	20	20	55.0	55.0	57.9	58.1	2.3	0.0	0.171651	70066	72096	80-2974	0.931034	87.9
clpP1_p5	clpP1	5	CGTTAACGCTACCAATGGGA	GCACCCCTTTTGTTCAATGG	20	20	50.0	50.0	58.0	57.8	16.0	0.0	0.181263	70066	72096	80-2869	0.931034	87.8
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GATCAAAGCGAGCCTCTTCA	AGGAAGAGATAAGAGGGCCC	20	20	50.0	55.0	58.0	57.9	0.0	16.5	0.126489	107591	107946	80-915	0.931034	88.2
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GATCAAAGCGAGCCTCTTCA	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.0	57.9	0.0	29.2	0.126489	107591	107946	80-914	0.931034	88.2
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GCGAGCCTCTTCATTCTGAA	AGGAAGAGATAAGAGGGCCC	20	20	50.0	55.0	58.0	57.9	0.0	16.5	0.126489	107591	107946	80-908	0.344828	64.8
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GCGAGCCTCTTCATTCTGAA	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.0	57.9	0.0	29.2	0.126489	107591	107946	80-907	0.344828	64.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	AAGCGAGCCTCTTCATTCTG	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.0	57.9	0.0	29.2	0.132108	107591	107946	80-909	0.344828	64.7
ycf1_p1	ycf1	1	GATCAAAGCGAGCCTCTTCA	AGAGAGTTGGCCGAGTTAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.6	0.089627	107947	109809	80-2552	0.310345	63.7
ycf1_p2	ycf1	2	CGGGAGAATCAATGACTGCA	AGAGAGTTGGCCGAGTTAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.6	0.102312	107947	109809	80-2779	0.310345	63.6
ycf1_p3	ycf1	3	ACGCCAGACTCGATCTCATA	AGAGAGTTGGCCGAGTTAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.6	0.105187	107947	109809	80-2425	0.310345	63.6
ycf1_p4	ycf1	4	GCGAGCCTCTTCATTCTGAA	AGAGAGTTGGCCGAGTTAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.6	0.089627	107947	109809	80-2545	0.206897	59.6
ycf1_p5	ycf1	5	AAGCGAGCCTCTTCATTCTG	AGAGAGTTGGCCGAGTTAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.6	0.095247	107947	109809	80-2547	0.206897	59.5
ndhF-rpl32_p1	ndhF-rpl32	1	GGGCGTGTAAACCAAAAACC	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.1	57.9	0.0	22.7	0.293402	112052	112814	80-1446	0.172414	51.0
ndhF-rpl32_p2	ndhF-rpl32	2	GGGGCGTGTAAACCAAAAAC	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.1	57.9	0.0	22.7	0.293402	112052	112814	80-1447	0.172414	51.0
ndhF-rpl32_p3	ndhF-rpl32	3	ATGAAGGGGAAATTGGGCAG	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.1	57.9	0.0	22.7	0.278851	112052	112814	80-1681	0.172414	50.8
ndhF-rpl32_p4	ndhF-rpl32	4	CACGAAAATCGAAACTGCCC	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.0	57.9	6.8	22.7	0.185169	112052	112814	80-1532	0.137931	47.2
ndhF-rpl32_p5	ndhF-rpl32	5	CACGAAAATCGAAACTGCCC	GCCCAATACCCCTTCTGTTT	20	20	50.0	50.0	58.0	57.8	6.8	0.0	0.267969	112052	112814	80-1462	0.068966	43.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAAAAGTACTCAAAACTTGGAA	CTCGCACTGCTTCCTAAGAG	22	20	27.3	55.0	52.3	58.1	0.0	0.0	7.742206	112989	113856	80-973	0.931034	49.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAAAGTACTCAAAACTTGGAA	CTCTCGCACTGCTTCCTAAG	22	20	27.3	55.0	52.3	58.1	0.0	0.0	7.742206	112989	113856	80-975	0.931034	49.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAAAGTACTCAAAACTTGGAA	GCTTCCTAAGAGCAACGTGT	22	20	27.3	50.0	52.3	58.2	0.0	0.0	7.865100	112989	113856	80-965	0.931034	49.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAAAGTACTCAAAACTTGGAA	TGCTTCCTAAGAGCAACGTG	22	20	27.3	50.0	52.3	58.2	0.0	0.0	7.866357	112989	113856	80-966	0.931034	49.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAAGTACTCAAAACTTGGAAT	CTCGCACTGCTTCCTAAGAG	22	20	27.3	55.0	52.1	58.1	0.0	0.0	7.969932	112989	113856	80-972	0.931034	49.2
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	AGGAAGAGATAAGAGGGCCC	GATCAAAGCGAGCCTCTTCA	20	20	55.0	50.0	57.9	58.0	16.5	0.0	0.126489	129341	129696	80-915	0.931034	88.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GGAAGAGATAAGAGGGCCCT	GATCAAAGCGAGCCTCTTCA	20	20	55.0	50.0	57.9	58.0	29.2	0.0	0.126489	129341	129696	80-914	0.931034	88.2
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	AGGAAGAGATAAGAGGGCCC	GCGAGCCTCTTCATTCTGAA	20	20	55.0	50.0	57.9	58.0	16.5	0.0	0.126489	129341	129696	80-908	0.344828	64.8
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GGAAGAGATAAGAGGGCCCT	GCGAGCCTCTTCATTCTGAA	20	20	55.0	50.0	57.9	58.0	29.2	0.0	0.126489	129341	129696	80-907	0.344828	64.8
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GGAAGAGATAAGAGGGCCCT	AAGCGAGCCTCTTCATTCTG	20	20	55.0	50.0	57.9	58.0	29.2	0.0	0.132108	129341	129696	80-909	0.344828	64.7