Hotspot

ycf1

IRb · 114696-114776 · 81 bp

Pi 0.169

MarkerSeek score 75.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 81
variable_sites: 18
pi: 0.169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	437-518 bp	1.0	87.8
ycf1_p2	CCGGAACTTCTTCGTAGTGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	437-518 bp	1.0	87.8
ycf1_p3	CCGGAACTTCTTCGTAGTGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	436-517 bp	1.0	87.8
ycf1_p4	GAGCTACTGAGGAACAACGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	533-614 bp	1.0	87.1
ycf1_p5	GAGCTACTGAGGAACAACGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	532-613 bp	1.0	87.1