Hotspot

rpl16

LSC · 86155-87716 · 1562 bp

Pi 0.00412

MarkerSeek score 49.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1562
variable_sites: 12
pi: 0.00412
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCGGCGATTACTAGCTCCT	ATGGATCCGAGAAGGTAGGG	50.0	55.0	58.1	58.0	2098-2107 bp	1.0	81.1
rpl16_p2	ATCGGCGATTACTAGCTCCT	CGAGGGAATCGCACGTATAG	50.0	55.0	58.1	58.0	2458-2467 bp	1.0	80.7
rpl16_p3	ATCGGCGATTACTAGCTCCT	ATCTTCTGGTGTCGAGGGAA	50.0	50.0	58.1	58.1	2470-2479 bp	1.0	80.5
rpl16_p4	ATCGGCGATTACTAGCTCCT	TCTTCTGGTGTCGAGGGAAT	50.0	50.0	58.1	58.1	2469-2478 bp	1.0	80.5
rpl16_p5	ATCGGCGATTACTAGCTCCT	TAGGCCACGAAAACTCGAAG	50.0	50.0	58.1	57.9	2368-2377 bp	1.0	80.4