Hotspot

petD

LSC · 81445-82684 · 1240 bp

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Pi 0.00282
MarkerSeek score 40.60
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1240
variable_sites
7
pi
0.00282
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 GTTGAAGGAATTCCCCGAAGA CTCCCTAGATACGCACATCA 47.6 50.0 57.9 56.2 1329 bp 0.25 27.3
petD_p2 AGTTGAAGGAATTCCCCGAAG CTCCCTAGATACGCACATCA 47.6 50.0 57.9 56.2 1330 bp 0.25 27.3
petD_p3 TGAAGGAATTCCCCGAAGAG CTCCCTAGATACGCACATCA 50.0 50.0 56.9 56.2 1327 bp 0.25 27.2
petD_p4 GTTGAAGGAATTCCCCGAAGA CCCTAGATACGCACATCATGA 47.6 47.6 57.9 57.1 1327 bp 0.25 25.9
petD_p5 GTTGAAGGAATTCCCCGAAGA TCCCTAGATACGCACATCATG 47.6 47.6 57.9 57.1 1328 bp 0.25 25.9