Hotspot

ndhF

SSC · 114886-117093 · 2208 bp

Pi 0.00507

MarkerSeek score 48.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2208
variable_sites: 22
pi: 0.00507
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCGGAACTTCTTCGTAGTG	TCGAAGAGAGAGTATGCCCA	55.0	50.0	58.0	57.6	2878-2962 bp	1.0	80.2
ndhF_p2	CCGGAACTTCTTCGTAGTGG	TCGAAGAGAGAGTATGCCCA	55.0	50.0	58.0	57.6	2877-2961 bp	1.0	80.2
ndhF_p3	GTAACCCCCGGAACTTCTTC	TCGAAGAGAGAGTATGCCCA	55.0	50.0	57.9	57.6	2884-2968 bp	1.0	79.4
ndhF_p4	CTCCGTTCCATGCCTCATTT	TCGAAGAGAGAGTATGCCCA	50.0	50.0	58.2	57.6	2857-2941 bp	1.0	78.3
ndhF_p5	TCCGTTCCATGCCTCATTTC	TCGAAGAGAGAGTATGCCCA	50.0	50.0	58.2	57.6	2856-2940 bp	1.0	78.3