Hotspot

ccsA-ndhD

SSC · 120509-120809 · 301 bp

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Pi 0.0296
MarkerSeek score 53.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
301
variable_sites
17
pi
0.0296
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTGGACCGTATTCGCGATTT TCCGGATTTCGTCCTCTCAT 45.0 50.0 58.0 58.2 538-552 bp 1.0 83.0
ccsA-ndhD_p2 TTGGACCGTATTCGCGATTT GGGCATGAGTGGTTTTGTTG 45.0 50.0 58.0 57.8 805-819 bp 1.0 82.0
ccsA-ndhD_p3 TTGGACCGTATTCGCGATTT TTATCTCGACGAAATGGGCG 45.0 50.0 58.0 58.2 895-909 bp 1.0 81.7
ccsA-ndhD_p4 TTGGACCGTATTCGCGATTT GCATGAGTGGTTTTGTTGCA 45.0 45.0 58.0 57.8 803-817 bp 1.0 81.5
ccsA-ndhD_p5 TTGGACCGTATTCGCGATTT CATATTTGCCCCCTGGTTGA 45.0 50.0 58.0 57.8 1150-1164 bp 1.0 81.0